Understanding the colloidal stability of protein therapeutics using dynamic light scattering
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چکیده
Dynamic light scattering (DLS) using noninvasive backscatter (NIBS) detection technology is capable of measuring the hydrodynamic radius of proteins over a large concentration range (0.1 mg/mL to 100 mg/mL). As a result, it is possible to determine, in actual biopharmaceutical formulations, the bulk viscosity/restricted diffusion interaction parameter (kD), particle interaction parameter (B22), melting temperature, onset temperature of protein aggregation and transition enthalpies to assess colloidal stability.
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